Genetic relationships of salep orchid species and gene flow among Serapias vomeracea x Anacamptis morio hybrids

Palaz E. B., DEMİREL F., Adali S., Demirel S., YILMAZ A.

PLANT BIOTECHNOLOGY REPORTS, cilt.17, sa.2, ss.315-327, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s11816-022-00782-w
  • Dergi Adı: PLANT BIOTECHNOLOGY REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, INSPEC, Veterinary Science Database
  • Sayfa Sayıları: ss.315-327
  • Anahtar Kelimeler: Crossing, Genetic conservation, iPBS, Genetic diversity, ENDANGERED MEDICINAL ORCHID, DENDROBIUM-NOBILE LINDL., CODON TARGETED SCOT, POPULATION-STRUCTURE, AMPLIFIED POLYMORPHISM, MOLECULAR CHARACTERIZATION, DIVERSITY ANALYSIS, IPBS, MARKERS, HETEROSIS
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Orchids are naturally grown in many countries of the Eastern Mediterranean. Salep, produced from orchid tubers via grinding and used as a hot drink, is an essential ingredient of ice cream. Salep orchid species are in danger of extinction due to the absence of cultivation and over-harvesting from nature. In this study, the genetic diversity and population structure between salep orchid species, their hybrids, and commercial species of Phalenopsis sp. were first investigated using inter-Primer Binding Site (iPBS) Retrotransposon markers. A total of 854 bands were scored with a 100% polymorphism rate. Neighbor-joining, model-based structure, and PCoA (Principal Coordinate Analysis) algorithms clustered the 30 salep orchids into three main populations. The analysis of molecular variance revealed variations within and among the populations as 71% and 29%, respectively. Anacamptis morio and Serapias vomeracea had the furthest genetic distances, and F-1 hybrids of S. vomeracea and A. morio had great genetic diversity. The study results will provide helpful information for orchid breeding by eliciting the genetic distances of salep orchids.