Effect of reduced production of aqueous humor on bacterial growth potential in rabbit eyes

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Batur M., Özer M. D., Tekin S.

19th Euretina Congress, Paris, France, 5 - 08 September 2019, pp.62

  • Publication Type: Conference Paper / Summary Text
  • City: Paris
  • Country: France
  • Page Numbers: pp.62
  • Van Yüzüncü Yıl University Affiliated: Yes


Purpose: To investigate the effects of anti-glaucomatous drops (reduce humor aqueous production) on clinical inflammation scores and bacterial growth rates in Staphylococcus aureus inoculated rabbit eyes.

                Methods: Fifteen New Zealand rabbits were used in the research. Left eyes (Group 1) of the rabbits selected as control (Placebo (Eyestil® hyaluronic acid)) and the right eyes (Group 2) were selected for research (Dorzolamide + Timolol (Cosopt® 20 mg/ml + 5 mg/ml). Eye drops were dropped into the eyes of each rabbit 12-hour intervals twice. The S.aureus (3750 CFU/0.05 ml) ATCC 6538 strain was injected into the anterior chamber of the right and left eyes two hours after the second drops. Two independent and blind investigators scored the clinical inflammation in the subsequent 24.,48. and 72. hours. Additionally, aqueous humor samples that were obtained during the follow-up were inoculated into pediatric blood culture media for bacterial growth analysis.

                Results: The total score of inflammation was higher in Dorzolamide+Timolole applied eyes comparing the controls. The significance rate was reached on the first day (p=0,04). The significance rate was lost in the following days, but still, the inflammation was more severe in group 2. There was no statistically significant difference in culture positivity between the groups on the first and second days. There was a significant difference on the third day (p = 0.03)

                Conclusions: Drugs that reduce humor aqueous production may increase the risk of endophthalmitis in the postoperative period by reducing aqueous humor clearance.