The Protective Effect Of Chrysin Against Glutamate-Induced Oxidative Stress, Inflammation And Apoptosis In C6 Glioma Cells

Ahlatcı A., Yıldızhan K.

VI. International Health Science and Life Congress (IHSLC2023), Burdur, Türkiye, 2 - 05 Mart 2023, ss.474

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Burdur
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.474
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

ABSTRACT

Background: Glutamate is one of the most important excitatory neurotransmitters released by nerve cells in the brain. Glutamate homoeostasis is essential for the regular functioning of the brain's functions. The literature has reported that high concentrations of glutamate are associated with diseases such as Parkinson's disease, Alzheimer's disease and Huntington's disease. Chrysin is a flavonoid in many plant extracts and renewable substances such as mushrooms, honey, and propolis. It has also been reported that chrysin has anti-inflammatory, anti-cancer and anti-oxidation properties. Aim: In our literature review, no study was found on the protective effect of chrysin in C6 glioma cells exposed to high glutamate concentrations. This study investigated the effect of chrysin against glutamate-induced excitotoxicity in C6 glioma cells. Methods: For the study, groups were formed from C6 cells as control, chrysin (50 μM, 24 h), Glutamate (10 mM, 24 h), and Glutamate+Chrysin. At the end of the study, total oxidant level (TOS), total antioxidant level (TAS), TNF-α, IL-1β, caspase-3 and caspase-9 levels in cells were measured with an ELISA kit. Results:  It was observed that exposure to high-dose glutamate (10 mM) in C6 cells increased TOS, TNF-α, IL-1β, caspase-3 and caspase-9, and decreased TAS levels (p<0.05). Furthermore, in the chrysin treatment group (Glutamate+Chrysin group), it was determined that, compared to the glutamate group, the chrysin increased TAS levels in cells and decreased TOS, TNF-α, IL-1β, caspase-3, and caspase-9 levels (p<0.05). Discussion and Conclusion: As a result, it was observed that chrysin has a protective effect on glutamate-induced excitotoxicity in the C6 cells. This study showed us that chrysin might be a protective factor in excitotoxic neuronal damage caused by glutamate.

Keywords: Glutamate, Chrysin, C6 glioma cells, Oxidative stress, Apoptosis