This investigation was conducted in order to determine the overall intracellular accumulation of Co2+, Cd2+ and Pb2+ on Bacillus cereus and Pseudonzonas aeruginosa which isolated from the soil samples collected from Van region. According to the results of the investigation, intracellular accumulation was observed to increase in wild type strains of B. cereus T 124 and P aeruginosa N 48 through the applications of Co2+, Cd2+ and Pb2+ (0.25, 0.5, 1, 2 and 4 mM). However, both the type of bacteria and the effect of various heavy metals caused different accumulation in two bacteria. In the three of the heavy metals put into application, B. cereus T 124 was found to have much more accumulation than P. aeruginosa N 48. The intracellular accumulation of Cd and Co metal was observed to be greater in B. cereus T 124 strain whereas the intracellular accumulation Pb2+ metal was seen to be much greater in P aeruginosa strain. The highest amount of accumulation was observed for Pb2+.