AIP ADVANCES, vol.1726, no.3, pp.300-305, 2016 (SCI-Expanded)
Lactococcosis, yersiniosis, listenollosis and cold water disease agents are frequently observed in Turkey as
bacterial fish pathogens. Bacterial fish pathogens have high mortality prognosis, causing significant economic losses for
the businesses. Use of molecular methods in substantiation of disease factors became prevalent in recent years. These
methods have a significant role in fast diagnosis and early treatment of fish diseases. In the present study, 8 rainbow
trout samples were obtained from each of 19 rainbow trout farms located in Van province, Turkey and registered with
Food, Agriculture and Livestock Ministry. Total genomic DNAs were isolated from kidney tissues of sampled rainbow
trout. Obtained DNAs were analyzed with real-time PCR there is/not (+/-) analysis using disease specific primer pairs
for each disease. Molecular diagnosis of lactococcosis pathogen in 4 farms out of 19 rainbow trout farms active in Van
province, and yersiniosis pathogen in 1 farm were made as a result real-time PCR analysis. Listenollosis and cold water
pathogens were not molecularly observed. Results of the present study demonstrated that the region was safe for
bacterial fish pathogens of cold water disease and listenollosis, which are observed frequently in Turkey, and there were
deficiencies in preventive measures against lactococcosis and yersiniosis and fish transfer was a significant reason for
the prevalence these diseases