Identification and phylogenetic differences of newly isolated Streptomyces sp.


Önalan Ş. , Seçkin H.

Turkish Journal of Agricultural and Natural Sciences, vol.8, no.3, pp.680-685, 2021 (International Refereed University Journal)

  • Publication Type: Article / Article
  • Volume: 8 Issue: 3
  • Publication Date: 2021
  • Title of Journal : Turkish Journal of Agricultural and Natural Sciences
  • Page Numbers: pp.680-685

Abstract


 Antimicrobial resistance and antibiotic use is a global health crisis today. Streptomyces is the source of most antimicrobials. Therefore, the similarities and differences within the species of Streptomyces are of great importance. In this study, isolation and identification of 3 different Streptomyces species isolated from sediment from the Dicle region were performed. Then, 16S rRNA gene sequence was obtained and the similarities and differences between species were revealed. As a result of the study, bacteria contained colorimetric differences, when looking at the spore chain morphology in the SEM image, a knobby structure was formed, 16S rRNA sequences longer than 1000 bp and in the phylogenetic tree created, 1 isolate was closely similar to the sequences obtained from GenBank (99 % <), other It was observed that the two isolates differed. 2 different Streptomyces isolates obtained in line with these results are important in terms of antibiotic production and different resistance conditions in subsequent studies.