3rd INTERNATIONAL CONFERENCE on FOOD, AGRICULTURE and VETERINARY 19-20 June, Izmir-TURKEY, 19 - 20 June 2021, pp.286-287
In this study, it was investigated biochemically, histopathologically and immunohistochemically whether it is possible to recycle the fibrosis by using nettle seed extract during the 2-month treatment process after the fibrosis was formed in the liver within an experimental period of 8 weeks with ethyl alcohol. A total of 24 rats were used in the study and the rats were divided into three groups, 8 in each group. The trial period was determined as 4 months in total. Control group; standard rat was fed with pellet feed. In the alcohol group and alcohol + nettle seed extract group, hepatic fibrosis was induced by giving 4 mL / kg 30% ethyl alcohol by orogastric gavage once a day in the first 2 months of the trial. In the next third and fourth months, only alcohol was given to the alcohol group, while the rats in the alcohol + nettle seed extract group were given nettle seed extract (30 mg / kg) together with alcohol and the recovery of fibrosis was observed at the end of the application. At the end of the experiment, blood and liver tissue samples taken from necropsied rats were examined biochemically, histopathologically and immunohistochemically. According to histopathological and immunohistochemical findings; It was observed that nettle seed extract recycled degenerative- necrotic changes and fibrosis in liver significantly thanks to its many bioactive components and therapeutic properties recorded in previous studies. In biochemical analysis; ALT, AST, LDH and HDL levels were decreased in the alcohol group compared to the control group (p <0.05). However, glucose, LDL and triglyceride levels were increased in the alcohol group compared to the control group (p <0.05). According to the alcohol group in the alcohol + nettle group; While ALT, LDH, LDL, glucose and triglyceride levels were decreased, alkaline phosphatase and HDL levels were increased compared to the alcohol group (p <0.05). The results of the study showed that nettle seed extract had antifibrotic effect in alcoholic hepatic fibrosis patients and significantly restored lipid profile and liver function tests. This research was supported by the Scientific Research Projects Directorate of Van Yüzüncü Yıl University as a projectnumbered TYL-2018-7586.