Species identification and germplasm conservation of origanum based on chloroplast genes

Furan M. A.

GENETIC RESOURCES AND CROP EVOLUTION AN INTERNATIONAL JOURNAL, vol.71, pp.1101-1112, 2024 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 71
  • Publication Date: 2024
  • Doi Number: 10.1007/s10722-023-01679-5
  • Journal Name: GENETIC RESOURCES AND CROP EVOLUTION AN INTERNATIONAL JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Geobase, Veterinary Science Database
  • Page Numbers: pp.1101-1112
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

DNA barcoding is a molecular approach for species identification and germplasm conservation in the Origanum genus. This study proposes a DNA- based method to accurately identify the numerous species within this diverse genus. Four chloroplast genes (matK, rbcL, rpoB, trnL) and a non-coding sequence (psbA) were analyzed. Among the applied barcoding loci, matK exhibited the longest aligned sequence length, followed by rpoB, rbcL, psbA-trnH, and trnL. The rbcL gene showed the highest number of informative sites, while psbA-trnH displayed the highest proportion of variable sites within the Origa- num genus. Phylogenetic analysis indicated that rbcL, rpoB, trnL, and psbA sequences were more effective than matK for identifying the Origanum genus, spe- cies, and subspecies. Combining multiple sequences improved accuracy. This study provides a molecular reference for herbal product identification and valu- able tools for species identification and germplasm conservation within the Lamiaceae family.