Akademik Veri Yönetim Sistemi
KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.27, sa.2, ss.307-315, 2024 (ESCI)
The Ziziphora species, classified under the Lamiaceae family, have a
strong aromatic property. Ziziphora species have been used in folk
medicine as sedative, gastric, aphrodisiac, bloating, and degassing. In the
current study, the phenolic and flavanoid content of ethanol extracts of
Ziziphora capitata L. species of flower, leaf, branch, mixed, and root parts
was determined by the LC-MS/MS device. In addition, the antioxidant
and cytotoxic activities of the extracts, as well as their inhibitory effects
on enzymes (antihypertensive, AchE (acetylcholinesterase), BchE
(butyrylcholinesterase), elastase, tyrosinase, collagenase and urease),
were determined. The LC-MS/MS results showed that quinic acid (25578,
5842, 25171, 14055, 10597 μg g-1, respectively) was found in higher
amounts in flower, leaf, branch, mixed, and root extracts of Z. capitata
species compared to other components. Additionally, rosmarinic acid
(17097 μg g-1), cynaroside (8432), and hesperidin (8067) were found to be
major components. It was observed that the flower extract of the species
exhibited strong antioxidant activity (IC50: 37.18±1.36 μg mL-1, 9.89±0.45,
A0.5:16.27±0.02, respectively) in DPPH, ABTS and CUPRAC methods. It
was concluded that the leaf extract of Z. capitata species had a strong
cytotoxic effect on HT-29 (colon cancer cell line) (viability %: 9.26±0.69).
It was observed that the root part of the species exhibited higher activity
in butyrylcholinesterase (BChE) enzyme inhibition activity (inhibition %:
40.56±0.88) than other parts. It was determined that Z. capitata extracts
did not show acetylcholinesterase, urease, tyrosinase, elastase,
collagenase, and antihypertensive enzyme activity or showed low
activity. As a result, it is thought that the flower extract of the Z. capitata
species has better results in terms of the examined parameters, whereas
the leaf extract needs to be subjected to more detailed in vitro and in vivo
research conducted to be used in the pharmaceutical industry as a result
of its cytotoxic effect against colon cancer cell lines.