Micro-spoon technique in estimation of sperm concentration in rat epididymis

Odabas O., Timurkan H., Atilla M., Yilmaz Y., Sengul E., Aydin S.

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, vol.191, no.1, pp.1-5, 2000 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 191 Issue: 1
  • Publication Date: 2000
  • Doi Number: 10.1620/tjem.191.1
  • Journal Name: TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1-5
  • Van Yüzüncü Yıl University Affiliated: No

Abstract

Several technical procedures mere described for estimation of epididymal sperm concentration until today. In all of the described techniques, epididymal material was drawn into a micro-pipette before counting procedure. We aimed to proceed this stage without using any pipette because of the difficulty in aspirating very viscous epididymal material into a micro-pipette. For measuring epididymal material we used a 2 mu l reservoir which me named as a micro-spoon. It was filled with the epididymal material and layered in a tube with Ringer's solution of 2 mi containing sodium hyaluronate. Then, it was mixed in order to obtain 1/1000 diluted suspension. Sperm cells in this suspension were counted on a Makler chamber under a light microscope. The procedure was easy and time saving, compared to the other techniques. (C) 2000 Tohoku University Medical Press.