2nd International Conference on Food, Agriculture and Animal Sciences, Antalya, Türkiye, 8 - 11 Kasım 2019, ss.193-202, (Tam Metin Bildiri)
The medicinal plant deve gülü which belongs to the Malvaceae family is used in the treatment of tonsilitis, gastric ulcers, pneumonia, excretory system infections and pelade. The aim of this study was to develop an in vitro germination method for deve gülü plant as a source of sterile explants in tissue culture studies. Three different sodium hypochlorite concentrations (5%, 7.5% and 10%), incubation at 42 C and nicking of the seed coat with scalpel were tested for germination. According to germination rate results, it was determined that incubation at 42 C did not affect germination rate. On the contrary, seeds damaged by scalpel germinated rapidly. Cotyledon, cotyledonary node and hypocotyl became available as explant source for tissue culture studies on the seventh day. Leaf formation started on the eighth day. Leaf, petiole and shoot tip explants were available to use in tissue culture on the thirtieth day. As a conclusion, an efficient germination method for deve gülü marshmallow having medicinal importance was developed with this study.