Diplotaenia turcica’s effect on liveliness on the NRK-52E cell line in different concentrations and periods


Çetin S., Yüksek V., Dede S., Usta A., Özdek U.

1. Euroasia Biochemical Approaches & Technologies Congress (1.EBAT-2018), Antalya, Turkey, 27 - 30 October 2018, vol.1, no.1, pp.55

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.55
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Aim: This research was planned to determine the various concentrations of the Diplotaenia Turcica extract,which is traditionally known to be good for diabetes, blood pressure, and rheumatism, to be used in several studies in cell culture. Material and Method: The NRK-52E cells were proliferated in an environment of 37oC, 5% CO2 and 95% of humidity, and in a RPMI 1640 DMEM media containing 1% penicillin/streptomycin 2 mM LGlutamine and 10% FBS. The cells were cultured in a plate of 96 as to be 104 . Diplotaenia Turcica plant was prepared with ethyl alcohol and water extract. The prepared Diplotaenia Turcica plant was applied to the renal cell line for 6,12 and 24 hours of time. Afterwards, the MTT assay was performed and liveliness percentage was determined. Findings: Different concentrations of Diplotaenia Turcica extract applied in the 6,12 and 24th hours had no effects on the cell liveliness under 50 µg/ml of concentrations and similar results to the control group was observed. 50 µg/ml concentration was observed to increase the cell proliferation in the 3rd hour by 20%, 40% in the 6th hour, and by the 24th hour resulted in a liveliness rate close to the control group and did not change the proliferation. But, all concentrations above 50 µg/ml were determined to cause cell death in all application periods and that these concentrations are cytotoxic. Result: It was found that concentrations over 50 µg/ml of Diplotaenia Turcica in future in vitro studies cannot be used as a useful dose for being cytotoxic but, 50 µg/ml and below are useful concentrations. Keywords: Diplotaenia Turcica, NRK-52E Cell, MTT