The Apoptotic, Cytotoxic, and Anti-migration Effects of Sodium Deoxycholate in a Breast Cancer Cell Line and its Modulation on PON1 as a Predictive Risk Marker.
Current molecular medicine, cilt.25, sa.8, ss.1057-1070, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 25 Sayı: 8
- Basım Tarihi: 2025
- Doi Numarası: 10.2174/0115665240312216241003060934
- Dergi Adı: Current molecular medicine
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
- Sayfa Sayıları: ss.1057-1070
- Anahtar Kelimeler: antimetastatic, Apoptosis, breast cancer, cytotoxicity, PON1 modulation, sodium deoxycholate
- Van Yüzüncü Yıl Üniversitesi Adresli: Evet
Özet
Abstract
Introduction: Breast cancer is the most prevalent cancer among women and is
usually treated with antineoplastic drugs. The present study examines the
influence of sodium deoxycholate on the molecular pathways underlying
apoptosis, cytotoxicity, and the modulation of PON1 in the MCF-7 breast cancer
cell line. Various doses were administered to test the hypothesis that it could
potentially affect cancer cells.
Methods: The study examined the cytotoxic effect of sodium deoxycholate on
MCF-7 cells and human mammary epithelial cells (CRL-4010) using the MTT method
to detect its anticancer properties. Subsequently, the efficacy of the active
dose on DNA fragmentation and apoptosis was examined using the apoptotic DNA
ladder and Western blot methods. Additionally, oxidative stress index and cell
migration tests were conducted. Notably, sodium deoxycholate did not cause DNA
damage despite demonstrating cytotoxic effects on cells.
Results: The study found that sodium deoxycholate increased the levels of
several pro-apoptotic proteins, leading to apoptosis. Moreover, it markedly
diminishes the activity of paraoxonase and arylesterase of PON1, which are
predictive risk markers for cancer. Furthermore, it was found to delay cell
migration in a time-dependent manner.
Conclusion: These findings suggest that sodium deoxycholate exhibits an antimetastatic effect in breast cancer cells, could be a valuable subject for further cancer research.
