IV. International Eurasian Agriculture and Natural Sciences Congress, Konya, Turkey, 30 - 31 October 2020, pp.103
Melon (Cucumis melo L.) is an important vegetable crops grown in different geographical regions of Turkey. Zucchini yellow mosaic virus (ZYMV), Cucumber mosaic virus (CMV) and Watermelon mosaic virus (WMV) cause major economic damages because of destructive effect on melon. Along with 22 different melon genotypes that are collected from the Lake Van Basin, 3 resistant genotypes and 2 commercial varieties were screened against these three viruses by mechanical inoculation and molecular methods. Firstly, in the study, virus inoculation was performed on melon genotypes using only ZYMV virus, and disease severity was determined according to 0-5 scale by performing morphological observations in genotypes. In second stage of the study the presence of markers related to resistance genes against to ZYMV, CMV and WMV were defined in these genotypes by screening with 7 primers including 3 SCAR primers and 4 CAPS primers. It has been determined that the disease severity was ranged between 10- 95% in melon genotypes. While bands were obtained from SCOPE14541 primer in 21 genotypes SCAPB051046 primer that was developed based on the Creb-2 resistance gene gave bands only in the U6, U13 and YYU6 genotypes from 25 genotypes. VirSq-F19 primer that was developed based on the resistance genes to ZYMV and WMV gave band in all genotypes excluding YYU47. Only two of the 4 CAPS primers associated with the Zym resistance gene produced band.