Deneysel Florozis Oluşturulmuş Ratların Böbrek Dokusunda C ve E Vitaminleri-nin İnflamasyon Gen Ekspresyonu Üzerine Etkisi


Van Sağlık Bilimleri Dergisi, vol.14, no.2, pp.199-208, 2021 (Peer-Reviewed Journal)


ABSTRACT Objective: Inflammation is an important step in elucidating fluorine-induced toxicity mechanisms. In this study, it was aimed to reveal the possible effects of the administration of vitamins C and E for the treatment of fluorosis nephrotoxicity on inflammation gene expressions. Material and Method: In the study, 48 male Wistar-Albino rats weighing 200-250 g were used. Six groups of 8 rats each were formed as control(K), Corn oil(M), NaF(NaF), NaF+vitamin E(NVE), NaF+vitamin C(NVC), NaF+vitamin C+vitamin E(NVCE). For 16 weeks, 150 mg/kg/day NaF was added to the drinking water of rats. Since vitamin E was dissolved in corn oil, the corn oil control group was formed and corn oil was given to rats 0.2 ml/day. After NaF was administered to treatment groups for 16 weeks, vitamin C (100mg/kg/day), vitamin E (300mg/kg/day), vitamin C+vitamin E (100 mg/kg/day+300mg/kg/day) were administered orally for 4 weeks. At the end of the experiment, the expression of inflammation markers IL-1β, TNF-α genes in RNA isolation products obtained from rat kidney tissue in all groups were determined by real time-qPCR. Beta-actin (Actb) was used as the internal control gene. Results: TNF-α expression levels were highest in NaF group. It was significantly lower than in all other groups. IL-1β gene expression levels; It was found lowest in corn oil and NaF + vitamin C + E groups and highest in NaF and NaF + vitamin C groups. Conclusion: In possible kidney damage caused by increased fluoride in the experimentally induced fluorosis with NaF, it was observed that the application of antioxidant vitamins for therapeutic purposes reduced inflammation. It was determined that vitamin C, vitamin E, vitamin C+vitamin E had significant beneficial effects on inflammation markers. It was observed that the combination of NaF+vitaminC+E was more effective in reducing inflammation compared to the groups given NaF+vitamin C and NaF+vitamin E. Keywords: Antioxidant vitamins, Fluorosis, Gene expression, Inflammation, Kidney.