Genetic variability of bovine herpesvirus type 4 (BoHV-4) field strains from Turkish cattle herds.

Bilge Dağalp S., Dogan F., Babaoglu A. R. , Aligholipour Farzani T., Alkan F.

Veterinaria italiana, vol.57, pp.49-59, 2021 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 57
  • Publication Date: 2021
  • Doi Number: 10.12834/vetit.2095.11150.1
  • Journal Name: Veterinaria italiana
  • Journal Indexes: Science Citation Index Expanded, Scopus, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
  • Page Numbers: pp.49-59
  • Keywords: Bovine herpesvirus type 4, Glycoprotein B (gB), PCR, Sequencing, Thymidine kinase (TK), Virus isolation, GLYCOPROTEIN-B, DAIRY-COWS, DNA, INFECTION, GENOME


Bovine herpesvirus type 4 (BoHV-4) is a common virus in the world that is detected in clinically ill or in apparently healthy cattle.This study provides a molecular characterization of BoHV-4 strains from 24 cattle some showing respiratory and/or reproductive problems and some without any apparent clinical sign. This study also reported the growth properties of five BoHV-4 field isolates. The 24 sampled cattle came from 13 different herds in 10 provinces collected between 2007 and 2018. Phylogeneticanalysis using partially amplified nucleotide sequences of ORF8 genes coding glycoprotein B (n = 24) and ORF3 genes coding thymidine kinase (n = 9), demonstrated genetic variability among the BoHV-4 strains analysed. The partial gB gene sequences clustered in three different genotypes (genotype 1,11and111) were located within the genotype I cluster, such as Movar strain. The analysis of the five BoHV-4 strains isolated from vaginal swabs (n = 2), nasal swab (n = 1), and brain samples (n = 2) revealed no significant differences in their growth properties in MDBK cell culture.