The aim of this study was to investigate the protective effect of Linum usitatissimum L. seed oil (LUSO) against ultraviolet C (UVC) exposure through monitoring some hematological and biochemical constituents of rats. For this purpose, rats were divided into three groups: control, UVC and UVC+LUSO. The rats of UVC and UVC+LUSO groups were subjected to UVC during 1 h twice a day for 4 weeks. LUSO (4 ml/kg b.w.) was given to rats of UVC+LUSO group by gavage before each irradiation period. Results showed that lipase and cholesterol levels were markedly higher in UVC group than control. Lipase decreased significantly, and cholesterol diminished slightly with combined treatment of UVC and LUSO. While the level of red cell distribution width (RDWc) decreased in UVC group, it increased significantly after LUSO supplementation. Apart from that, calcium and monocyte levels increased markedly in UVC+LUSO group compared with control and UVC groups. Significant decreases were determined in amounts of white blood corpuscles and lymphocytes as well as an increase was observed in monocyte percentage of UVC+LUSO group compared to control. This investigation showed that treatment of LUSO with UVC supplied relative protection against UVC injury and perfected some blood parameters significantly, even when compared with control group.