A combination of omega-3 and exercise reduces experimental Achilles tendinopathy induced with a type-1 collagenase in rats

Gundogdu G., Tasci S. Y., Gundogdu K., TERİM KAPAKİN K. A., DEMİRKAYA A. K., Nalcı K. A., ...More

Applied Physiology, Nutrition and Metabolism, vol.48, no.1, pp.62-73, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 48 Issue: 1
  • Publication Date: 2023
  • Doi Number: 10.1139/apnm-2021-0801
  • Journal Name: Applied Physiology, Nutrition and Metabolism
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, CINAHL, EMBASE, MEDLINE, SportDiscus, Veterinary Science Database
  • Page Numbers: pp.62-73
  • Keywords: Achilles tendinopathy, exercise, omega-3, rat
  • Van Yüzüncü Yıl University Affiliated: Yes


© 2022 The Author(s).This study aimed to evaluate the effectiveness of omega-3 supplementation with exercise in a collagenase-induced Achilles tendinopathy (AT) rat model. Experimental groups (healthy control (HC), AT, exercise (Ex), omega-3 (W), and Ex+W) were randomly allocated. After a week of adaptation, oral omega-3 was initiated for 8 weeks (5 days/week). The exercise groups performed treadmill running for 30 min/day (5 days/week, 20 m/min, 8 weeks) following one week of adaptation (10 m/min, 15 min/day). Matrix metalloproteinase-13 (MMP-13), interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and total antioxidant–oxidant status (TAS) levels were determined in serum samples. Tendon samples were obtained for biomechanical, histopathological, and immunohistochemical assessments. Ultimate tensile force, yield force, stiffness values, collagen type-I alpha 1 expression, and serum TAS significantly decreased (P < 0.05) in AT vs. HC. These values and expression significantly increased in the Ex+W group vs. AT. Serum MMP-13, IL-1β, and TNF-α levels decreased in all treatment groups vs. AT. The most significant decrease was found in the Ex+W group (P < 0.01). Histopathologically, the improvement in degeneration was statistically significant in the Ex+W group (P < 0.05). Immunohistochemically, MMP-13, IL-1β, TNF-α, and nitric oxide synthase-2 expression was decreased in all treatment groups vs. AT. In conclusion, omega-3 and exercise might be recommended in AT patients.