1. A batch of wheat distillers dried grains with solubles (DDGS) was obtained immediately after production and was separated into 5 equal parts and placed in woven polypropylene sacks. The samples were stored under 5 different temperature conditions for 1 year as follows: kept at a constant -20 degrees C; kept at -20 degrees C for 24h period and after that kept at a constant +4 degrees C; kept at a constant +4 degrees C only; kept at a constant +15 degrees C; stored at ambient temperature (range of weekly mean temperatures was from +4 to +22 degrees C).2. Each of the 5 wheat DDGS samples was included (200g/kg) in a nutritionally complete diet and fed to broiler chickens from 7 to 21 d of age. The chemical composition of the DDGS samples was determined at the beginning and at the end of the 1-year storage period.3. The nitrogen corrected apparent metabolisable energy (AMEn) and the nutrient availability of each sample was measured using a total collection technique. The growth performance of birds was also determined.4. The DDGS samples kept at a constant -20 degrees C had higher dry matter, lower oxidation value and lower antioxidant contents. The DDGS sample that was stored at ambient temperatures had a higher AMEn than the rest of the DDGS samples.5. The results of this experiment have shown that there can be changes in the AMEn of wheat DDGS during storage at ambient temperatures. In general, there were no serious effects of storage of DDGS on its feeding value to broiler chickens.