Brucella Coombs gel test: Can it replace other serological methods as a rapid test in serological diagnosis of brucellosis? Brucella Coombs jel testi: Brusellozun serolojik tanısında hızlı bir test olarak diğer serolojik yöntemlerin yerini alabilir mi?


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Erdin B. N. , Parlak M., Aydinli A. G. , Demir F.

Turk Hijyen ve Deneysel Biyoloji Dergisi, vol.78, no.3, pp.249-254, 2021 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 78 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.5505/turkhijyen.2021.83584
  • Journal Name: Turk Hijyen ve Deneysel Biyoloji Dergisi
  • Journal Indexes: Scopus, Academic Search Premier, CAB Abstracts, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.249-254
  • Keywords: Brucella, brucella coombs gel test, brucella coombs jel testi, brucellacapt, brucellacapt, Brusella, coombs, coombs, standart tube agglutination, standart tüp aglutinasyon
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

© 2021. All Rights Reserved.Objective: The aim of the study was to compare the results of Brucella Coombs gel test (BCGT) with Standart tube agglutination test (STA), Coombs anti-Brucella test (CAB), Brucellacapt test (CAPT) for the serological diagnosis of brucellosis. Methods: A total of 180 blood samples, were included in the study and were simultaneously tested with STA, CAB, CAPT and BCGT. Results of the tests were evaluated in terms of diagnostic performance and Kappa coefficient. Results: When the tests were compared to CAB test, which is a reference method in the serological diagnosis of the brucellosis, in terms of diagnostic performance; the highest sensitivity (98.1%) and accuracy (98.9%) was found with BCGT. When tests were evaluated in terms of Kappa coefficient, BCGT had the highest value (0.796) and substantial agreement with CAB. Conclusion: In conclusion, when compared with CAB, BCGT showed substantial agreement and highest sensitivity and accuracy. In addition, reduction of the period for test results and elimination of the need for experienced staff makes this test superior to other methods. Although BCGT seems to replace other tests in the serological diagnosis of brucellosis, its sensitivity and specificity should be put forward in culture-confirmed cases and controls with a comprehensive study.