Oxidative DNA damage in patients with cataract

Ates O., Alp H. H., KOÇER İ., BAYKAL O., Salman I. A.

Acta Ophthalmologica, vol.88, no.8, pp.891-895, 2010 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 88 Issue: 8
  • Publication Date: 2010
  • Doi Number: 10.1111/j.1755-3768.2009.01583.x
  • Journal Name: Acta Ophthalmologica
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.891-895
  • Keywords: cataract, malondialdehyde, oxidative DNA damage, oxidative stress
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Purpose: This study examines the levels of oxidative damage in patients with cataract. Methods: Blood samples were collected from 60 patients with cataract and 60 age- and gender-matched healthy individuals to measure 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels. Results: A significant difference was observed in leukocyte 8-OHdG levels in patients with cataract in comparison with healthy persons (p < 0.001). Similarly, a significant difference was observed in plasma MDA levels in patients with cataract in comparison with healthy persons (p < 0.001). In addition, a significant correlation was found between levels of 8-OHdG in leukocyte DNA and plasma MDA (r = 0.859, p < 0.001). Conclusion: This study measured the oxidative DNA damage by measuring the 8-OHdG in the leukocyte DNA in patients with cataract. In addition, the level of MDA - a marker for lipid peroxidation - was measured to determine lipid peroxidation. Journal compilation © 2009 Acta Ophthalmol.