Prevalance of Giardia duodenalis in Anatolian Buffalos Calves in Kütahya Region in Turkey


Ayan A. , Erol U., İleri M., Oruç Kılınç Ö., Oktay Ayan ., Öner A., ...More

International Congress (Webinar) on Life Sciences Life Sciences Virtual 2021, Pennsylvania, United States Of America, 27 - 28 January 2021, pp.31

  • Publication Type: Conference Paper / Summary Text
  • City: Pennsylvania
  • Country: United States Of America
  • Page Numbers: pp.31

Abstract

Giardia duodenalis is a unicellular flagellate parasite that infects the intestinal tract of a variety of mammals, including humans.The life cycle of this protozoan is direct, cysts are eliminated in the feces and are infective when ingested. Transmission occurs bythe fecal-oral route or by ingestion of contaminated food and water. The aim of this study was to investigate the prevalence of G. duodenalis in Anatolian Buffalos in the Kütahya region in Turkey. In this study A total of 80 Anatolian buffalos calves fecal samples which were up to 6 months of age were collected some farms in Kütahya region in Turkey during 2019-2020 and brought to the laboratory. After that, they were examined in terms of Giardia spp. cysts under microscope by applying native examination method. Then DNA extraction was performed according to the kit protocol by using QIAamp Fast DNA Stool Mini Kit. Nested PCR was performed with appropriate primers. The obtained PCR products were run on 1.5% agarose gel stained with RedSafe™ Nucleic Acid Staining Solution and images were obtained using gel imaging device. Microscopically, Giardia spp. cysts were found in 25 out of 80 (%31,25) examined samples. As a result of the Nested PCR, 30 (%37,5) samples were found positive in terms of Giardia duodenalis. It was determined that Giardia duodenalis is quite commonly observed in Anatolian buffalos calves in the Kütahya region in Turkey. Moreover, it was suggested that further precautions be taken.