DETERMINATION OF THE OCCURRENCE FREQUENCY OF CRYPTOSPORIDIUM SP. IN CHILDREN BROUGHT TO THE HOSPITAL WITH THE COMPLAINT OF DIARRHEA USING DIFFERENT METHODS

Ekici, A.; Unlu, Ahmet; Halidi, A.; Aydemir, Selahattin; Yilmaz, H.

doi:10.15666/aeer/1904_29052913

DETERMINATION OF THE OCCURRENCE FREQUENCY OF CRYPTOSPORIDIUM SP. IN CHILDREN BROUGHT TO THE HOSPITAL WITH THE COMPLAINT OF DIARRHEA USING DIFFERENT METHODS

Atıf İçin Kopyala

Ekici A., Unlu A. H., Halidi A. G., Aydemir S., Yilmaz H.

APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, cilt.19, sa.4, ss.2905-2913, 2021 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 19 Sayı: 4
Basım Tarihi: 2021
Doi Numarası: 10.15666/aeer/1904_29052913
Dergi Adı: APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Pollution Abstracts, Veterinary Science Database
Sayfa Sayıları: ss.2905-2913
Anahtar Kelimeler: cryptosporidiosis, children, diarrhea, immunochromatographic test, nested PCR, SPP.
Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Cryptosporidium is a parasite responsible for diarrhea in humans. Practitioners rarely routinely request Cryptosporidium diagnostic tests; thus, its prevalence is likely underrated. The prevalence of cryptosporidiosis among children brought to the hospital with the complaint of diarrhea was investigated using 3 different methods, comprising nested PCR, immunochromatographic testing, and microscopic examination with modified acid-fast staining. Cryptosporidium sp. was detected in 42/150 children with diarrhea (28%) and in 1/50 children in the control group (2%). The main complaint by children admitted to hospital with Cryptosporidium was diarrhea. Additionally, some clinical symptoms/signs like abdominal pain, nausea, vomiting, stool mucus, weakness, weight loss, and anorexia were correlated with cryptosporidiosis. Results from the 3 methods were compared and nested PCR and immunochromatographic testing were the most reliable. Among other pathogens and parasites found in stool samples, Cryptosporidium is a significant cause of hospitalization due to gastrointestinal disease in children in Van, Turkey.