In this study, Walnut seedlings (Juglans regia L.) were grafted on dormancy period and placed into controlled conditions with 26 +/- 2 degrees C temperature and 60-70% humidity. IAA changes in shoot of graft, root and graft area were measured in 1, 14, 21, 28, 35, 42 and 49th days following grafting by high performance liquid chromatography (HPLC). IAA levels in the shoot of graft was maximum (251 ng/g) at the 21 st day, and minimum (59 ng/g) at the 49 th days. The levels of IAA of graft area were maximum (218 ng/g) at the 14 th day, and minimum (53 ng/g) at the 49th days. IAA concentration in roots were highest (213 ng/g) at the 14 th day later and minimum (42 ng/g) at the 49 th day. IAA levels showed rapid increase for 3 weeks in the shoot of graft and for 2 weeks in the root, and the graft area. IAA concentration had the decreasing and parallel tendency with each other after 21 th day.