Investigation of Babesia spp. in Stray Dogs in Van Province by Polymerase Chain Reaction


Selçin Ö., Oğuz B.

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi, vol.8, no.2, pp.156-161, 2022 (Peer-Reviewed Journal)

Abstract

Objective: Canine babesiosis is a tick-borne protozoal disease caused by Babesia spp., which can cause hemolytic anemia, splenomegaly, thrombocytopenia, and fever. As a result of molecular studies conducted in Turkey, the presence of B. canis, B. gibsoni, B. vogeli, B. rossi, and B. vulpes species has been revealed. To the best of our knowledge, there are no studies detecting Babesia species in dogs of Van province. This study was carried out to investigate the presence and prevalence of babesiosis in dogs of Van province using the Polymerase chain reaction (PCR) technique. Methods: Blood samples were taken from vena cephalica antebrachii into EDTA tubes from a total of 100 randomly selected asymptomatic dogs. DNAs obtained from the samples were investigated by the PCR method in which 18S ribosomal RNA gene was amplified for the presence of Babesia spp. Results: Babesia spp. DNA was not found in any of the 100 stray dogs examined according to PCR results. Conclusion: In this study, Babesia spp. species were investigated molecularly for the first time in stray dogs of Van region.