ISPEC 10th International Conference On Engineering & Natural Sciences , Siirt, Turkey, 16 - 18 May 2021, pp.464
ABIOTIC STRESS FACTORS AND SIGNAL MOLECULES AFFECT SECONDARY METABOLITES COMPOSITON OF ST. JOHN’S WORT BOTH IN VIVO AND IN VITRO
İN VİVO VE İN VİTRO ŞARTLARDA YETİŞTİRİLEN SARI KANTARON BİTKİSİNDE ABİYOTİK STRES FAKTÖRLERİ VE SİNYAL MOLEKÜLLERİNİN SEKONDER METABOLİT KOMPOZİSYONUNA ETKİLERİ
Neşe ERAY VURAN
Res. Asist., Van Yüzüncü Yıl University, Faculty of Science, Department of Molecular Biology and Genetic, email@example.com (Corresponding Author), Orcid ID: 0000-0001-6387-1493
Assoc. Prof. Dr., Van Yüzüncü Yıl University, Faculty of Pharmacy, firstname.lastname@example.org, Orcid ID: 0000-0002-0080-2519
Prof. Dr., Yildiz Technical University, Faculty of Engineering, Department of Bioengineering, email@example.com, Orcid ID: 0000-0003-3195-1119
In the present study, Hypericum perforatum L. was regenerated in vitro and abiotic stress factors and signal molecules were applied on in vitro regenerants to investigate the change of phenolic compounds concentration comparing to plants collected from field. The effects of media composition, explant type and Plant Growth Regulators (PGRs) in different combination and concentration on callus production, plant regeneration and secondary metabolite production were evaluated. The quantitative analysis of phenolic composition was performed by High Performance Liquid Chromatography (HPLC).
According to the results, MS medium supplemented with 1 mg/L 2, 4-D + 1 mg/L BA was determined to be the best medium for callus formation in dark. When micro propagation is considered, MS medium supplemented with 2 mg/L NAA + 1 mg/L BA was the best PGRs combination. Multiple shoots developed in MS supplemented with 2 mg/L NAA + 1 mg/L BA were separated from each other and rooted in hormone-free MS medium. The leaf explant gave the highest shoot number. The shoot explant produced the highest amount of callus.
The effects of abiotic stress factors such as drought and UV-C and some signal molecules such as ascorbic acid, salicylic acid, sucrose and different PGRs on the production of phenolic compounds were investigated. It was demonstrated that callus was able to produce hypericin and pseudohypericin compounds. B5 and SH media gave better results than MS medium for the production of hypericin and pseudohypericin. The combination of NAA + BA was detected the best PGRs on phenolic compounds production. Chlorogenic acid concentration was increased by UV and sucrose, quercetin was increased by ascorbic acid and pseudohypericin was increased by drought applications compared to the control. The increases in the amounts of phenolic compounds were statistically significant (p <0.01). Rutin was not detected in the plants grown in vivo and in vitro. However, the plant grown in laboratory conditions produced rutin compound under drought stress.
Keywords: Abiotic stress, Hypericum perforatum L., Phenolic compound, Signal Molecule, Tissue culture