Akademik Veri Yönetim Sistemi
ISPEC 10th International Conference On Engineering & Natural Sciences , Siirt, Türkiye, 16 - 18 Mayıs 2021, ss.464
ABIOTIC STRESS
FACTORS AND SIGNAL MOLECULES AFFECT SECONDARY METABOLITES COMPOSITON OF ST.
JOHN’S WORT BOTH IN VIVO AND IN VITRO
İN VİVO VE İN VİTRO ŞARTLARDA YETİŞTİRİLEN SARI
KANTARON BİTKİSİNDE ABİYOTİK STRES FAKTÖRLERİ VE SİNYAL MOLEKÜLLERİNİN SEKONDER
METABOLİT KOMPOZİSYONUNA ETKİLERİ
Neşe ERAY VURAN
Res. Asist., Van Yüzüncü Yıl University, Faculty of Science, Department
of Molecular Biology and Genetic, neseeray@yyu.edu.tr (Corresponding
Author), Orcid ID: 0000-0001-6387-1493
Abdullah DALAR
Assoc. Prof. Dr., Van Yüzüncü Yıl University, Faculty of Pharmacy, dalar.abdullah@yyu.edu.tr, Orcid
ID: 0000-0002-0080-2519
Musa TÜRKER
Prof. Dr., Yildiz Technical University, Faculty of
Engineering, Department of Bioengineering, musaturker@yahoo.com,
Orcid ID: 0000-0003-3195-1119
In
the present study, Hypericum perforatum
L. was regenerated in vitro and abiotic
stress factors and signal molecules were applied on in vitro regenerants to
investigate the change of phenolic compounds concentration comparing to plants
collected from field. The effects of media composition, explant type and Plant
Growth Regulators (PGRs) in different combination and concentration on callus
production, plant regeneration and secondary metabolite production were
evaluated. The quantitative analysis of phenolic composition was performed by
High Performance Liquid Chromatography (HPLC).
According
to the results, MS medium supplemented with 1 mg/L 2, 4-D + 1 mg/L BA was
determined to be the best medium for callus formation in dark. When micro propagation
is considered, MS medium supplemented with 2 mg/L NAA + 1 mg/L BA was the best PGRs
combination. Multiple shoots developed in MS supplemented with 2 mg/L NAA + 1
mg/L BA were separated from each other and rooted in hormone-free MS medium. The
leaf explant gave the highest shoot number. The shoot explant produced the
highest amount of callus.
The
effects of abiotic stress factors such as drought and UV-C and some signal
molecules such as ascorbic acid, salicylic acid, sucrose and different PGRs on
the production of phenolic compounds were investigated.
It was demonstrated that callus was able to produce
hypericin and pseudohypericin compounds. B5 and SH media gave better results
than MS medium for the production of hypericin and pseudohypericin. The
combination of NAA + BA was detected the best PGRs on phenolic compounds
production. Chlorogenic acid concentration
was increased by UV and sucrose, quercetin was increased by ascorbic acid and
pseudohypericin was increased
by drought applications compared to the control. The increases in the amounts of phenolic compounds were
statistically significant (p <0.01). Rutin was not detected in the plants
grown in vivo and in vitro. However, the plant grown in
laboratory conditions produced rutin compound under drought stress.
Keywords:
Abiotic stress, Hypericum perforatum
L., Phenolic compound, Signal Molecule, Tissue culture