Green synthesis of silver nanoparticles (AgNPs) was performed using extracts from Ornithogalum narbonense L. (ONL) as a cost-effective and eco-friendly approach. A surface plasmon resonance peak was observed at 440 nm after 12 h. The intense reddish-brown coloration was observed higher than 1.5 mM AgNO3 concentrations. Bioactive compounds were found responsible for the reduction, stabilization, and capping of Ag+ to AgNPs. X-ray powder diffraction analysis confirmed the face-centered cubic silver crystalline structure. The average spherical-shaped individual particle size was around 8.46 nm, while hydrodynamic size ranged between 7-13 nm and 36-131 nm. The AgNPs have good stability (zeta = -31.15 mV). Energy-dispersive X-ray spectroscopy analysis proved the presence of elemental Ag (6.76%). The antibacterial activity with the zone of inhibition is as follows: P. aeruginosa > E. coli > S.aureus > B. cereus (10.35 +/- 0.12 mm > 9.40 +/- 0.14 mm > 8.20 +/- 0.10 mm > 7.0 +/- 0.24 mm). The AgNPs did not show antifungal properties against C. albicans.