Serum Anti-Müllerian Hormone Levels during Estrus and Diestrus Phases of the Estrous Cycle and its Possible Effect on Fertility in Cross-bred Hamdani Sheep
PAKISTAN VETERINARY JOURNAL, sa.0253-8318, ss.1-6, 2024 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2024
- Doi Numarası: 10.29261/pakvetj/2024.134
- Dergi Adı: PAKISTAN VETERINARY JOURNAL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
- Sayfa Sayıları: ss.1-6
- Van Yüzüncü Yıl Üniversitesi Adresli: Evet
Özet
This study aimed to assess the variations in serum Anti-müllerian hormone (AMH) levels during the estrus and diestrus phases of the estrous cycle and its effect on fertility in cross-bred Hamdani ewes. In the study, 21 non-prolific cross-bred Hamdani ewes (aged 2-3 years with BCS 2.75-3.25) were subjected to estrous synchronization using intra-vaginal sponges. Following synchronization, ewes underwent natural mating and pregnancy examination was carried out on the 45th day after mating through trans-rectal ultrasonography. Jugular blood samples were collected on day of estrus and 8 days later and analyzed for serum AMH and progesterone concentrations. Results showed that serum AMH levels were 100-480 and 95-520 pg/ml during estrus and diestrus phases, respectively. There was no change in serum AMH levels due to age, BCS and during estrus and diestrus phases of the estrous cycle (P>0.05). Estrus and diestrus serum AMH levels were significantly higher in pregnant ewes compared to non-pregnant ewes (P<0.05). Receiver-operating characteristic (ROC) analysis revealed that serum AMH cut-off value of >270 pg/mL was feasible to predict fertility in cross-bred Hamdani ewes. In conclusion, serum AMH can be used as a marker of fertility in non-prolific cross-bred Hamdani ewes.