Akademik Veri Yönetim Sistemi
1st International Veterinary Biochemistry and Clinical Biochemistry Congress (1.VBCB-2018), Hatay, Türkiye, 12 - 15 Nisan 2018, cilt.1, sa.1, ss.217-218
Aim: This study was planned in order to effect of lycopene (LYC) known pro
inflammatory and antioxidant properties the potential oxidative DNA damage(8-
hydroxy-2'-deoxyguanosine, 8-OHdG) and reveals the effects of antioxidant system
in high glucose (HG) added kidney cell line.
Material and Methods: BHK-21 cells were cultured with the regular passages (%5
FBS, %10 host serum, %1 L-Glutamine, %1 penicilin/ streptomicin/ %0.0125
gentamicin- RPMI 1640, %5CO2 ve %95, 37oC incubation). MTT cell viability
was determined for glucose and LYC. Cells were seed into plates. Control, glucose
(285 mM), LYC (50 µM) and its cross groups were prepared. After twenty-four
hour incubation, trypsinized cells were disrupted by freeze / thaw method and
analyzed. In these samples; 8-OhdG, total antioxidant status (TAS), total oxidant
status (TOS) and oxidative stress index were determined.
Results: 8-OHdG levels were effected by high glucose (p≤0.05) and, HG plus LYC
supplements were increased. TOS levels and OSI values in all experimental groups
(HG, LYC and LYC+HG) increased compared to controls (p≤0.05). HG treatment
was decreased TAS in LYC groups (p≤0.05).
Conclusion: In the HG treated BHK-21 cells, oxidative DNA damage, TOS, OSI
increased compared to the control for all treated groups. Oxidative DNA damage
and OSI were higher than HG, and, HG plus LYC groups. It was determined that
the dose of LYC used in this study and the duration of administration caused an
increase in oxidative stability with high glucose. It will be useful to plan for further
study at different times, doses and with new parameters to understand the
mechanism and causes of this elevation.
Keywords: DNA damage, lycopene, TAS/TOS, high glucose