The effect of lycopene on oxidative DNA damage and antioxidant system on high glucose treated renal cell line


Dede S., Yur F., Taşpınar M., Çetin S., Usta A., Yüksek V.

1st International Veterinary Biochemistry and Clinical Biochemistry Congress (1.VBCB-2018), Hatay, Türkiye, 12 - 15 Nisan 2018, cilt.1, sa.1, ss.217-218

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası: 1
  • Basıldığı Şehir: Hatay
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.217-218
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Aim: This study was planned in order to effect of lycopene (LYC) known pro inflammatory and antioxidant properties the potential oxidative DNA damage(8- hydroxy-2'-deoxyguanosine, 8-OHdG) and reveals the effects of antioxidant system in high glucose (HG) added kidney cell line. Material and Methods: BHK-21 cells were cultured with the regular passages (%5 FBS, %10 host serum, %1 L-Glutamine, %1 penicilin/ streptomicin/ %0.0125 gentamicin- RPMI 1640, %5CO2 ve %95, 37oC incubation). MTT cell viability was determined for glucose and LYC. Cells were seed into plates. Control, glucose (285 mM), LYC (50 µM) and its cross groups were prepared. After twenty-four hour incubation, trypsinized cells were disrupted by freeze / thaw method and analyzed. In these samples; 8-OhdG, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index were determined. Results: 8-OHdG levels were effected by high glucose (p≤0.05) and, HG plus LYC supplements were increased. TOS levels and OSI values in all experimental groups (HG, LYC and LYC+HG) increased compared to controls (p≤0.05). HG treatment was decreased TAS in LYC groups (p≤0.05). Conclusion: In the HG treated BHK-21 cells, oxidative DNA damage, TOS, OSI increased compared to the control for all treated groups. Oxidative DNA damage and OSI were higher than HG, and, HG plus LYC groups. It was determined that the dose of LYC used in this study and the duration of administration caused an increase in oxidative stability with high glucose. It will be useful to plan for further study at different times, doses and with new parameters to understand the mechanism and causes of this elevation. Keywords: DNA damage, lycopene, TAS/TOS, high glucose