Akademik Veri Yönetim Sistemi
Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, cilt.11, sa.2, ss.574-585, 2022 (Hakemli Dergi)
The present study
aimed to evaluate the fatty acid and phenolic profiles, and antioxidant and
antibacterial activities of A. rupicola.
For this purpose, For this purpose, the chemical content of the methanol
extract was first determined using the HPLC-TOF/MS method. Thereafter, five
extracts having different polarities (hexane-hexane, hexane-chloroform,
methanol-hexane, methanol-chloroform and methanol-butanol) were obtained using
the partitioning method, and the chemical contents of these extracts were determined
by the GC-MS method. According to these data, various fatty acids and phenolic
contents were observed. Furthermore, the antioxidant studies were performed
with total phenolic compounds and ABTS, while antibacterial studies were
performed with the microdilution test and the disc diffusion test using three
bacteria, one gram-positive and two gram-negative bacteria. The antioxidant and
antibacterial tests revealed that A.
rupicola is both an antioxidant and an antibacterial plant. According to
the obtained results, the microdilution test was shown to be more effective
than the disc diffusion test. On the other hand, the inhibition percent vs.
extract concentration graph showed that the MH was the most effective
inhibitor. In this study, it was revealed that the methanol extract and five
extracts from A. rupicola had a variety of phenolic compositions and
fatty acids, respectively, and these five extracts also possess antioxidant
effects and antibacterial activities.