Neurotoxic responses in brain tissues of rainbow trout exposed to imidacloprid pesticide: Assessment of 8-hydroxy-2-deoxyguanosine activity, oxidative stress and acetylcholinesterase activity

TOPAL A., ALAK G., ÖZKARACA M., Yeltekin A. Ç., ÇOMAKLI S., Acil G., ...Daha Fazla

CHEMOSPHERE, cilt.175, ss.186-191, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 175
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.chemosphere.2017.02.047
  • Dergi Adı: CHEMOSPHERE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.186-191
  • Anahtar Kelimeler: Imidacloprid, Pesticide, Oxidative stress, 8-OHdG, Acetylcholinesterase, Fish, Brain, Toxicity, MESSENGER-RNA EXPRESSION, DNA-DAMAGE, HISTOLOGICAL ALTERATIONS, COMPARATIVE TOXICITY, ONCORHYNCHUS-MYKISS, LIPID-PEROXIDATION, OXYGEN RADICALS, ANTIOXIDANT, BIOMARKERS, ENZYMES
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

The extensive use of imidacloprid, a neonicotinoid insecticide, causes undesirable toxicity in non targeted organisms including fish in aquatic environments. We investigated neurotoxic responses by observing 8-hydroxy-2-deoxyguanosine (8-OHdG) activity, oxidative stress and acetylcholinesterase (AChE) activity in rainbow trout brain tissue after 21 days of imidacloprid exposure at levels of (5 mg/L, 10 mg/L, 20 mg/L). The obtained results indicated that 8-OHdG activity did not change in fish exposed to 5 mg/L of imidacloprid, but 10 mg/L and 20 mg/L of imidacloprid significantly increased 8-OHdG activity compared to the control (p < 0.05). An immunopositiv reaction to 8-OHdG was detected in brain tissues. The brain tissues indicated a significant increase in antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)) compared to the control and there was a significant increase in malondialdehyde (MDA) levels (p < 0.05). High concentrations of imidacloprid caused a significant decrease in AChE enzyme activity (p < 0.05). These results suggested that imidacloprid can be neurotoxic to fish by promoting AChE inhibition, an increase in 8-OHdG activity and changes in oxidative stress parameters. Therefore, these data may reflect one of the molecular pathways that play a role in imidacloprid toxicity. (C) 2017 Elsevier Ltd. All rights reserved.