In this study, we aimed to examine the effect of volatile oil extracts of Helichrysum plicatum DC (HP) and Tanacetum balsamita L. (TB) on the histopathology of the testicles of the mice that had alloxan-induced type 1 diabetes or with imitated physiopathology of type 2 diabetes. The volatile oil extracts of HP and TB; were injected intra. peritoneally in diabetic mice at 12.5, 25 and 50 mg kg(-1) doses. Control and reference groups received 0.2 mL serum physiologic intra peritoneally and oral glibenclamide at 0.3 mL kg(-1) dose, respectively. Blood samples were withdrawn from tail veins of the mice 1, 2 and 24 It after the treatment and the blood glucose levels were measured. We investigated testicular histopathology of the mice in control and diabetic groups. The examination of the testicles of diabetic mice revealed reduction in diameters of the seminiferous tubules and thickening in the wall of the seminiferous tubules in addition to degenerative changes and decline in the number of spermatogenic cells. There were multinucleated giant cells in the lumens of some seminiferous tubules. No significant shown was detected in the severity of lesions between control group of diabetic mice receiving serum physiologic and the diabetic mice treated with the extracts of H? and TB. TUNEL-positive cells were higher diabetic mice than in control mice. We monitored no marked encouraging effect of HP and TB volatile oil extracts on the testicular histopathology. The reason for this failure might be due to short term use of these extracts. Long term applications of volatile oil extracts of HP and TB at various doses remained to be done to elucidate the potential anti-diabetic effect of these extracts.