2nd İnternational conference on "veterinary biochemistry and clinical biochemistry (2.VBCB-2019), Ankara, Turkey, 24 - 26 October 2019, vol.2, no.4, pp.169-172
This study was planned to investigate the possible protective effect of Vitamin C on the ionized radiation induced
necrotic and autophagicpathways as in vitro. The NRK-52E renal epithelial cells were cultured (RPMI 1640 medium
(10% FBS. 1% L-Glutamine (2mM) and 1% Penicillin/Streptomycin). The proliferation-enhancing concentration of
Vitamin C (100 µM) was used. In this study 5 groups were designed (Control, Ionizing Radiation, Vitamin C, Ionizing
Radiation+Vitamin C, Vitamin C+Ionizing Radiation). The ionizing radiation groups were exposed 8 Gy ionizing radiation.
Total RNA and cDNA were isolated from the samples collected from the incubated cells for 24 hours, andexpression
levels of target genes were determined by RT-PCR. According to the control gene by RT-PCR; the expression levels of
necrotic (RIPK 1, RIPK3) and autophagic (ATG3, ATG5, BECN1, Map3A) target genes were determined. In the radiation
group, necrotic and autophagic genes were found to be significantly increased compared to control group. The
vitamin C supplementation after radiation decreased genes expression of all genes except Map3A gene. As a result, it
was noted that ionizing radiation affects necrotic and autophagic cell pathways in the NRK-52E cell line, and activate
cytotoxic mechanism. Vitamin C leads to down regulation for necrotic and autophagic genes, except for Map3A
before and after ionized radiation administration. It was considered that the effects of vitamin C on cytotoxicity due
to ionizing radiation are worth investigating.
Key words: Autophagy, In vivo, Ionizing radiation, Necrosis, Vitamin C