International Biochemistry Congress – 2017 /28 th National Biochemistry Congress (TurkJBiochem-2017), Erzurum, Turkey, 19 - 23 September 2017, vol.42, no.1, pp.36
OBJECTIVES: The present study was planned to investigate the cellular damage
in osteoblast cell lines in the fluoride metabolism that are most likely to be
effected by toxicity and the roles of certain minerals (Ca, Se, Al, Mg) inoxidant /
antioxidant mechanisms that are likely to occur in prevention of the
MATERIALS-METHODS: Cells were propagated in vitro with two to three
regular passages per week. MTT viability test and the NaF IC50 value were
determined with non-toxic mineral doses. Cells were cultured in 96-well plates.
For samples used in TAS and TOS analysis, cells were harvested with
trypsinization following the 24-hour incubation and prepared for the assay by
disintegration with the freeze / thaw method. TAS and TOS were determined by
ELISA test using a commercial kit.
RESULTS: TAS decreased in selenium administered groups (p≤0.05), but the
decrease in Naf administered group was insignificant. In the Mg +NaF
administered group, TAS levels were the highest (p ≤ 0.05). TOS levels were the
highest in the NaF-administered group (p≤0.05), and decreased in all NaF +
mineral administered groups (p≤0.05) and approached the control levels.
CONCLUSION: It was observed that the decreased TAS, increased TOS, and
OSI levels in the osteoblast cell line with NaF administration approached the
control group levels after the administration of the minerals. It was concluded that
the cell viability in the osteoblast cell line was consistent with the TAS / TOS
Keywords: Cell culture, NaF, oxidative stress index, minerals, osteoblast