The effect of certain minerals on the total oxidant/antioxidant system in sodium fluoride (NaF) induced osteoblast cell line


Çetin S., Kömüroğlü A. U. , Yur F. G. , Taşpınar M., Usta A., Yüksek V.

International Biochemistry Congress – 2017 /28 th National Biochemistry Congress (TurkJBiochem-2017), Erzurum, Turkey, 19 - 23 September 2017, vol.42, no.1, pp.36

  • Publication Type: Conference Paper / Summary Text
  • Volume: 42
  • City: Erzurum
  • Country: Turkey
  • Page Numbers: pp.36

Abstract

OBJECTIVES: The present study was planned to investigate the cellular damage in osteoblast cell lines in the fluoride metabolism that are most likely to be effected by toxicity and the roles of certain minerals (Ca, Se, Al, Mg) inoxidant / antioxidant mechanisms that are likely to occur in prevention of the abovementioned mechanisms. MATERIALS-METHODS: Cells were propagated in vitro with two to three regular passages per week. MTT viability test and the NaF IC50 value were determined with non-toxic mineral doses. Cells were cultured in 96-well plates. For samples used in TAS and TOS analysis, cells were harvested with trypsinization following the 24-hour incubation and prepared for the assay by disintegration with the freeze / thaw method. TAS and TOS were determined by ELISA test using a commercial kit. RESULTS: TAS decreased in selenium administered groups (p≤0.05), but the decrease in Naf administered group was insignificant. In the Mg +NaF administered group, TAS levels were the highest (p ≤ 0.05). TOS levels were the highest in the NaF-administered group (p≤0.05), and decreased in all NaF + mineral administered groups (p≤0.05) and approached the control levels. CONCLUSION: It was observed that the decreased TAS, increased TOS, and OSI levels in the osteoblast cell line with NaF administration approached the control group levels after the administration of the minerals. It was concluded that the cell viability in the osteoblast cell line was consistent with the TAS / TOS balance. Keywords: Cell culture, NaF, oxidative stress index, minerals, osteoblast