In this study, the isolation of C. sordellii from abomasum lesions of lambs and the confirmation of the isolates by polymerase chain reaction (PCR) was attempted. A total of 298 abomasal specimens (swabs) obtained from lambs of 4 to 8 months of age were investigated. Sampled swabs were inoculated onto blood agar plates and incubated anaerobically. Following the incubation grown colonies were identified according to their morphological and biochemical characteristics. C. sordellii was isolated from 17 of 298 specimens (5.7%). These bacteria were also confirmed to harbour C. sordellii DNA by PCR. In conclusion, the presence of C. sordellii in abomasum lesions was demonstrated in Turkey for the first time with this study.