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Gürbüz F. N., Koçdoğan A. K., Yılmaz C., Oğuztüzün S., Güler Şimşek G., Türk M.
International Journal of Nature and Life Sciences, vol.6, no.1, pp.25-40, 2022 (Peer-Reviewed Journal)
Abstract
Multidrugresistance is an important factor limiting the effect of
chemotherapy on cancer treatment. Disorders of drug transport and
apoptosis, deterioration of redox homeostasis are among the main
mechanisms that lead to multidrug resistance. The aim of this study was
to determine the effect of 5-FU on GST isozymes, drug resistance
proteins and apoptotic proteins before and after 5-Flourouracil
application on DLD-1 colon cancer cell line. The cytotoxic effect of
5-FU was measured by WST-1test and, the efficiency of drug application
was, also, proved by double staining via Hoechst 33342 with Propidium
iodide. Next, the expression levels of GST isozymes, drug resistance
proteins and apoptotic proteins were determined by immunocytochemistry.
The cytotoxic effect of 5-FU at different doses on DLD-1 colon cancer
cell line was determined by WST-1 method. MRP-2, 3, 6, 7 of drug
resistance proteins; GSTA1, GSTM1, GSTT1, GSTZ1, GSTK1 and GSTO1 of GST
proteins; bcl-2, caspase-3, p38, and p53, which are apoptotic proteins,
have higher expression in the drug-treated DLD-1 cell line. GSTS1, MDR-1
and MRP-1expressions were not immunocytochemically different. It was
determined that there is a direct correlation between the level of
cytotoxicity and applied drug concentration. The cytotoxic effect of the
drug increased with the increase in the dose of the drug. In this
study, as first in the literature, the expression levels of some
apoptotic markers, GST isozymes and drug resistance proteinswere
evaluated togetherand except GSTS1, MDR-1 and MRP-1, they were all
upregulated with respect to the control group after 5-FU administration.