Akademik Veri Yönetim Sistemi
Turkish Journal of Veterinary Research (Online), cilt.4, sa.2, ss.45-49, 2020 (Hakemli Dergi)
ABSTRACT
Objective: The aim of this study was to determine the effects of lycopene administration as a protective
agent against necrotic damage of NaF, a fluoride compound found to have high cytotoxic effects in the renal
epithelial cell.
Material- Method: The renal epithelial cell was cultured in DMEM high glucose medium, containing
10%FBS, 1%L-Glutamine (2mM) and 1% penicillin/streptomycin. With the MTT viability test, the non-toxic
dose of lycopene (1 µM) and the IC50 value of NaF at the 24th hour was determined to be 3200 µM. The study
groups were divided into four as control, NaF, lycopene and NaF+lycopene (the combination of NaF and
lycopene). After the total mRNA obtained from these groups were converted to cDNA, expression levels of
the identified necrotic genes were determined by real-time PCR method.
Results: While the Ripk1 gene did not change in the group given lycopene at the 24th hour, it was found
that it increased 2.6 times in the group that received only fluoride, while it increased 7 times in the group
treated with NaF+lycopene. A significant difference was detected between the groups in terms of gene
expression pattern. While the Ripk3 gene increased slightly in the 24th hour applied lycopene group, it was
observed that only NaF applied group increased 8 times and NaF+lycopene applied group increased in the
9 times.
Conclusion: Based on the results obtained from this study, it was seen that activation of necrotic genes is
important in explaining the molecular basis of cell death from NaF, which is applied as fluoride source, in
revealing the molecular basis of the necrotic pathway. It was found that the decrease in cell viability due to
NaF increased with lycopene, but the use of lycopene with fluoride also increased necrotic gene expression.
Keywords: NaF, in vitro, Lycopene, Necrotic Genes