In this study, the effect of experimental administration of fluorine and DMBA, alone or in combination, on rats on vascular responses was investigated. After adaptation, the rats were randomly selected and divided into 9 groups, 10 male rats in each group. While sodium fluoride was applied daily in drinking water for 12 weeks to the groups to which fluoride will be applied; on the other hand, DMBA was dissolved in sesame oil and applied to the groups that needed it, once a week for a total of 12 weeks. The groups were determined as C, SO, 1NaF, 15NaF, 30NaF, DMBA, 1NaF+DMBA, 15NaF+DMBA and 30NaF+DMBA. Tensions in the thoracic aorta were provided with phenylephrine. Then, relaxation responses were recorded by giving ACh ((10-8'-7'-6'-5)) and SNP ((10-,-9,-8,-7,)(-6)(,)(-5)) at different cumulative concentrations. In the study, it was determined that fluorine caused the vessels to contract and DMBA to relax. Concurrent exposure to high amounts of fluorine and DMBA was observed to cause vasoconstriction.