IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION
Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), cilt.10, sa.2, ss.132-137, 2021 (TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 10 Sayı: 2
- Basım Tarihi: 2021
- Doi Numarası: 10.18038/aubtdc.802794
- Dergi Adı: Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online)
- Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.132-137
- Van Yüzüncü Yıl Üniversitesi Adresli: Evet
Özet
This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.