Stinging Nettle Seed Effects on Some Cytokines in Rats Fed with High Fat Diet

Kömüroğlü A. U., Yaman T., Meydan İ., Uyar A., Çibuk S., Kaçak K.

IV İnternational Academic Research Congress, Antalya, Turkey, 27 - 30 October 2018, pp.135

  • Publication Type: Conference Paper / Summary Text
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.135
  • Van Yüzüncü Yıl University Affiliated: Yes


In this study, we aimed to investigate the effect of stinging nettle seed on some cytokines in rats fed with high fat diet.

32 rats were divided into 4 groups with 8 rats in each group; Control group, high-fat diet group (HFD), high-fat diet + stinging nettle seed group (HFDSNS) and stinging nettle seed (SNS) group. The feed containing high fat diet was prepared by mixing normal pellet (45%) with butter. HFDSNS and SNS groups were given orogastric gavage with the stinging nettle seed extract 300 mg / kg. At the end of the 10-week study, rats were sacrificed by cervical dislocation and blood was collected by intracardiac route. Serums of blood were separated. Serum IL-4, IL-6, IL-10 and TNF-α were studied by ELISA. endothelial nitric oxide synthase (eNOS) reactivity was examined as Immunohistochemically in liver tissue.

IL-10 and TNF levels were significantly higher in the HFD group than in the control group. TNF α and IL-10 levels were significantly lower in the HFDSNS group compared to the SNS group. eNOS immune-expression was observed in cynosoidal endothelial cells, Kuppfer cells / macrophages and vascular endothelial cells, whereas negative results were obtained in hepotocytes. The eNOS reactivity was found to be higher in the HFD group than in the control group.

SNS can be effective in preventing inflammation caused by HFD. Thus SNS can be a promising candidate for treatment of obesity.