Active substance production at the nano-level attracts attention in the scientific world due to its wide application area. Different methods are used for the biosynthesis of nanoparticles. Recent studies have focused on non-toxic, environmentally friendly synthesis methods. Nanoparticles obtained by green synthesis using various biological elements such as plants, microorganisms and proteins have taken part in many scientific studies. Plants, which have an important potential in active ingredient production, are highly preferred in nanoparticle production. Scanning Electron Microscope and Energy Dispersive X-Ray Analysis (SEM / SEM-EDX), Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD) and Ultraviolet visible light absorption Spectroscopy (UV-vis) techniques were used for the structural and morphological characterization of Zn nanoparticles obtained by green synthesis using Helichrysum arenarium plant extract and ZnO.The antioxidant capacity of Zn NPs/Ha structures was determined by performing the DPPH test. Antimicrobial effects of zinc nanoparticles on six different pathogens (Bacillus cereus ATCC 10876, Escherichia coli ATCC 25952, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATTC 29213, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATTC 90028) were investigated. As a result of this studies, it has been observed that it has an inhibitory effect against some pathogen microorganisms. It has also been found that its antioxidant content is at a significant level.