Effect of Heat Shock Treatment on Microspore Embryogenesis in Brassica oleracea Species

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Tuncer B., Cig A., Yanmaz R., Yaşar F.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.22, sa.4, ss.548-554, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Sayı: 4
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1501/tarimbil_0000001413
  • Dergi Adı: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.548-554
  • Anahtar Kelimeler: Embryo, Genotype, High temperature, Microspore culture, White cabbage, Kale, CULTURE, REGENERATION, TEMPERATURE, DESICCATION
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment (32 degrees C and 35 degrees C for 2 days) on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), three genotypes of Turkish kale (Balkaya, Yanmaz, Karadere 077) and five commercial F-1 ornamental kale hybrids (Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome). Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F-1 at 32 degrees C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 degrees C.