Effect of Heat Shock Treatment on Microspore Embryogenesis in Brassica oleracea Species

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Tuncer B., Cig A., Yanmaz R., Yaşar F.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, vol.22, no.4, pp.548-554, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 4
  • Publication Date: 2016
  • Doi Number: 10.1501/tarimbil_0000001413
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.548-554
  • Keywords: Embryo, Genotype, High temperature, Microspore culture, White cabbage, Kale, CULTURE, REGENERATION, TEMPERATURE, DESICCATION
  • Van Yüzüncü Yıl University Affiliated: Yes


Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment (32 degrees C and 35 degrees C for 2 days) on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), three genotypes of Turkish kale (Balkaya, Yanmaz, Karadere 077) and five commercial F-1 ornamental kale hybrids (Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome). Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F-1 at 32 degrees C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 degrees C.