Akademik Veri Yönetim Sistemi
Applied Fruit Science, cilt.67, sa.3, 2025 (Scopus)
In vitro modeling of plant responses to ultraviolet B (UV-B) stress is a multifaceted and critical research area in plant biology, genetics, biochemistry, and agricultural sciences. This modeling not only expands fundamental scientific knowledge but also serves as an indispensable tool for developing practical solutions aimed at sustainability and plant health in agriculture. This study was conducted to determine the physiological and biochemical responses of vines to UV‑B stress. ‘Erciş’ which is the autochthonous grape variety of Van, Türkiye, and its region grown at an altitude of 1725 m, with a history of three thousand years, and ‘Merlot’ grape variety, which has been grown in the Bordeaux region of France since the 18th century and is one of the most important red wine grape varieties in the world, were used as plant material. Explants required for in vitro studies were prepared from shoots obtained by maintaining wood cuttings of both grape varieties under controlled conditions. Single-node micro-cutting explants were cultured in MS nutrient medium containing 1 mg L−1 BAP. Plantlets aged 45 days had at least six full leaves and staggered into a plant growth pot were exposed to low (16 h ≅ 8.25 μW cm−2) and high (4 h ≅ 33 μW cm−2) UV‑B irradiation from a height of 40 cm in a cabinet. Control plantlets were incubated under cold white fluorescent lamps for only 16 h. Total phenolic compound (TP), antioxidant activity (AA), antioxidant enzymes (CAT, SOD, APX), and lipid peroxidation level (malondialdehyde [MDA]) were determined as physiological and biochemical responses to UV‑B stress. By the application of low UV‑B irradiation, the highest total phenolic compound and antioxidant activity were observed in the ‘Merlot’ grape variety with 45.72 mg GAE g−1 and 79.79 μmol TE mg−1, respectively. As a result of the study, although differences were observed between the varieties, their TP, AA, SOD, CAT, APX and MDA contents varied under UV‑B stress. The results of the study are important in terms of developing plant varieties resistant to UV‑B stress, increasing agricultural productivity and preserving the quality and quantity of plant products.