Akademik Veri Yönetim Sistemi
BİLTEK-IX INTERNATIONAL SYMPOSIUM ON CURRENT DEVELOPMENTS IN SCIENCE, TECHNOLOGY AND SOCIAL SCIENCES, Hakkari, Türkiye, 31 Mayıs - 02 Haziran 2024, ss.945-952
The aim of this study was to evaluate the chemical characterization and
the anti-ageing and anti-inflammatory activities of extracts prepared from the
aerial part of Nepeta betonicifolia CA Meyer. The plant
material was harvested from Gevaş district, Van province, Turkey, in June 2021.
The aerial parts of the plant were macerated separately with n-hexane, ethyl acetate and methanol
solvents. The tyrosinase, elastase and cyclooxygenase (COX) inhibitory
activities of the extracts were tested in
vitro. Phytochemical analysis of the
extracts was conducted using liquid chromatography-mass spectrometry/mass
spectrometry (LC-MS/MS) with 56 standards.. All samples significantly inhibited
the tyrosinase enzyme at concentrations of 100-500 µg/mL as follows: n-hexane extract,
46.78±0.35-68.57±1.95%, methanol extract, 28.03±3.30-84.54±3.25%, and ethyl
acetate extract, 27.34±1.45-97.50±2.50%. While the extracts showed no
inhibitory effect on elastase at a concentration of 100 µg/mL, the inhibitory
effect of the extracts at a concentration of 500 µg/mL was as follows; methanol
extract, 46.33±3.26%, ethyl acetate extract, 59.55±4.87%, and n-hexane extract, 94.95±1.15%. The all
extracts showed less than 5% inhibitory effect on COX (cyclooxygenase) enzymes
at a concentration of 100 µg/mL. The main compounds detected in the extracts
were cosmosiin, rosmarinic acid, luteolin-7-glucoside, chlorogenic acid and
quinic acid. To our knowledge, the inhibitory effect of Nepeta betonicifolia on
tyrosinase, elastase and COX enzymes has been reported for the first time in
this study.