Phytochemical and biological investigations on two Nepeta species: Nepeta heliotropifolia and N. congesta subsp. cryptantha


Akdeniz M., ERTAŞ A., Yener I., Fırat M., Kolak U.

JOURNAL OF FOOD BIOCHEMISTRY, vol.44, no.2, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 2
  • Publication Date: 2020
  • Doi Number: 10.1111/jfbc.13124
  • Journal Name: JOURNAL OF FOOD BIOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Business Source Elite, Business Source Premier, CAB Abstracts, Compendex, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Keywords: aroma, biological activities, essential oil, LC-MS, MS, Nepeta, phenolic compound, ESSENTIAL OIL COMPOSITION, ANTIOXIDANT ACTIVITIES, CHEMICAL-COMPOSITION, CATARIA L., CONSTITUENTS, LAMIACEAE, PROFILE, TYROSINASE, EXTRACTS, REGIONS
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

In the present study, the essential oil and aroma compositions of Nepeta heliotropifolia (NH) and N. congesta subsp. cryptantha (NC) were determined by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC/FID), and their phenolic compounds by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, antioxidant, cytotoxic, anticholinesterase, urease, and tyrosinase activities of essential oils obtained from NH and NC aerial parts and ethanol extracts prepared from different parts of NH and NC were investigated. The major constituent of water-distilled essential oils was found to be germacrene D (36.7% and 38.5%, respectively), and their main aroma component was eucalyptol (48.0% and 24.7%, respectively). Among the studied parts of NH and NC, their flowers extracts were found to be the richest in phenolic compounds and in which the most abundant compound was rosmarinic acid (8,909.91 and 4,317.20 mu g/g, respectively). NH and NC flower extracts exhibited also strong antioxidant activity in DPPH, ABTS, and CUPRAC assays. Among the tested samples, NH essential oil indicated the best cytotoxic effect against PDF, HT-29, and MCF-7 (IC50 52.34, 25.89, and 44.70 mu g/ml, respectively), and the highest butyrylcholinesterase (77.21 +/- 1.12% inhibition) and moderate acetylcholinesterase (41.36 +/- 0.69% inhibition) inhibitory activities.