INVESTIGATION AND MOLECULAR CHARACTERIZATION of Barley Yellow Dwarf Viruses in WHEAT FIELDS (Triticum aestivum L.) of DIYARBAKIR PROVINCE by MULTIPLEX RT- PCR


HASSAN N. O. , Usta M. , Al-Maaroof E. M. , GÜLLER A.

International Agricultural Sciences Congress, Van, Türkiye, 9 - 12 Mayıs 2018, ss.470

  • Basıldığı Şehir: Van
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.470

Özet

In order to ascertain the presence of Barley/Cereal yellow dwarf viruses (BYDV-PAV, MAV, SGV, RMV, and CYDV-RPV), in wheat fields, a survey was conducted in the year of 2016 in Diyarbakir province. Presence of BYDV viruses (PAV, MAV, SGV, RMV, and CYDV-RPV) was investigated by using multiplex RT-PCR and RT-PCR methods. A total of 365 wheat leaf samples were randomly collected from wheat fields of Diyarbakir province. As a result of tests the wheat samples were found to be infected by BYDV-PAV, BYDV-SGV and CYDV-RPV with the percentage of (3.5%), (2.4%) and (1.3%), respectively. Detected mix infections of BYDV-PAV+BYDV-SGV, BYDV-PAV+ CYDV-RPV, BYDV-SGV+ CYDV–RPV, and BYDV-PAV+ BYDV-SGV+ CYDV-RPV were 2.2%, 0.8%, 0.8%, and 0.8% respectively.

A virus isolate was selected randomly and its coat protein gene was characterized. Complete coat protein gene of BYDV-PAV virus (Genbank accession no. KX774424) was cloned and sequenced with the universal primers. BYDV-PAV Diyarbakir isolate was compared with 21 isolates from other regions in the world. The nucleotide sequence of BYDV-PAV Diyarbakir isolate was showed 81.43%- 98.3% similarities with the world isolates.

The present study is the first report for documentation of BYDV-PAV, BYDV-SGV and CYDV-RPV viruses in wheat fields in Diyarbakir province in Turkey.

 

Keywords: Wheat, viral diseases, Multiplex RT-PCR, Characterization, Cloning, Turkey.