Approximately 30% of plant species of Turkey, which is among the richest countries in terms of biodiversity, has been endemic. Wild rhubarb (Rheum ribes L.) is a wild vegetable grows especially in the eastern region of Turkey and is an endemic species. In this study, genetic relationships among 80 wild rhubarb genotypes collected from some regions of Lake Van Basin, which are in the distribution area, were tried to be determined by iPBS marker system. At the same time, a commercial variety of R. rhabarbarum, which is a cultivated species, was used as control. PCR studies were conducted with 23 iPBS primers to determine genetic relationships, and a total of 340 scorable bands were obtained. 100% polymorphism rate was obtained from all primers studied. While the average PIC value was found to be 0.90, the highest value was found to be 0.97 from the primer # 2220. It was determined that the genotypes were divided into 3 basic groups in the dendogram created with UPGMA based on Jaccard similarity coefficient.