The Teaching-Learning Based Optimization (TLBO) algorithm is a population-based optimization technique that has been shown to be competitive against other population-based algorithms. The main purpose of this paper is to solve the balancing problem of mixed-model two-sided assembly lines by using TLBO algorithm first time in the literature. Most recently, hybrid teaching-learning-based optimization (HTLBO) algorithm is proposed by  for solving the balancing of stochastic simple two-sided assembly line problem. The HTBLO algorithm is compared with the well-known 10 different meta-heuristic algorithms in the literature in . The tests performed underlined that HTLBO algorithm presented more outstanding performance when compared to other algorithms. In this paper, HTLBO algorithm is also adapted for solving the problem of balancing mixed-model two-sided assembly line and its performance is analysed. The objective function of this study is to minimize the number of mated-stations and total number of stations for a predefined cycle time. A comprehensive computational study is conducted on a set of test problems that are taken from the literature and the performance of the algorithms are compared with existing approaches. Experimental results show that TLBO algorithm has a noticeable potential against to the best-known heuristic algorithms and HTLBO algorithm results show that it performs well as far as the best-known heuristic algorithms for the problem in the literature.