Purificiation and some properties of polyphenol oxidase from van apple (golden delicious)

DEMIR H., Yoruk I., Savran A., Ekici K.

ASIAN JOURNAL OF CHEMISTRY, cilt.18, sa.1, ss.475-480, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 1
  • Basım Tarihi: 2006
  • Dergi Adı: ASIAN JOURNAL OF CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.475-480
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Polyphenol oxidase was isolated from van apple, with a 4.0-fold purification of polyphenol oxidase by ammonium sulfate and DEAE cellulose being achieved. The optimum pH was 4 and for this enzyme, the optimum temperature was 50 degrees C. V-max and K-m values were found of Lineweaver-Burk graphs. Potassium cyanide, benzoic acid, citric acid, MnCl2, NaCl, NaNO2 and CUSO4 were used as inhibitors, The enzyme activity was inhibited by benzoic acid, CUSO4 and NaCl, polyphenol oxidase activity at 10 mM, whereas NaNO2, citric acid, KCN and MnCl2 enhanced by polyphenol oxidase activity at 10 mM.