Separation of retinol acetate, vitamin A palmitate, alpha- and gamma-tocopherol by reverse-phase high performance liquid chromatography and determination of these vitamins in Van Lake fish (Chalcalburnus tarichi)


Testereci H., Yoruk I., Akyuz A., Sagmanligil H.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, vol.21, no.1, pp.17-22, 1997 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 1997
  • Journal Name: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.17-22
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

The resolution properties of several commercial columns for vitamin A and E standards by reverse phase chromatography methods have been examined, Hypersil MOS column with methanol-water (96:4) and acetonitrile-water (90:10) mobile phase, Shimadzu-ODS column with methanol-water (96:4) mobile phase have acceptable resoluion in separation of vitamin A esters. Shimadzu-ODS column/96% methanol and Shimadzu-C8/90% acetonitrile have the acceptable resolution ratio for vitamin E isomers.